Enter the directory of the maca folder on your drive and the name of the tissue you want to analyze.

tissue_of_interest = "Marrow"

Load the requisite packages and some additional helper functions.

library(here)
library(useful)
library(Seurat)
library(dplyr)
library(Matrix)
save_dir = here('00_data_ingest', 'tissue_robj')
# read the metadata to get the plates we want
plate_metadata_filename = here('00_data_ingest', 'facs_raw_data', 'metadata_FACS.csv')
plate_metadata <- read.csv(plate_metadata_filename, sep=",", header = TRUE)
colnames(plate_metadata)[1] <- "plate.barcode"
plate_metadata

Subset the metadata on the tissue.

tissue_plates = filter(plate_metadata, tissue == tissue_of_interest)[,c('plate.barcode','tissue','subtissue','mouse.sex')]
tissue_plates

Load the read count data.

#Load the gene names and set the metadata columns by opening the first file
filename = here('00_data_ingest', 'facs_raw_data', 'FACS', paste0(tissue_of_interest, '-counts.csv'))
raw.data = read.csv(filename, sep=",", row.names=1)
# raw.data = data.frame(row.names = rownames(raw.data))
corner(raw.data)

Make a vector of plate barcodes for each cell

plate.barcodes = lapply(colnames(raw.data), function(x) strsplit(strsplit(x, "_")[[1]][1], '.', fixed=TRUE)[[1]][2])
head(plate.barcodes)
[[1]]
[1] "D042044"

[[2]]
[1] "D042044"

[[3]]
[1] "D042044"

[[4]]
[1] "D042044"

[[5]]
[1] "D042044"

[[6]]
[1] "D042044"

Use only the metadata rows corresponding to Bladder plates. Make a plate barcode dataframe to “expand” the per-plate metadata to be per-cell.

barcode.df = t.data.frame(as.data.frame(plate.barcodes))
rownames(barcode.df) = colnames(raw.data)
colnames(barcode.df) = c('plate.barcode')
head(barcode.df)
                      plate.barcode
A22.D042044.3_9_M.1.1 "D042044"    
C5.D042044.3_9_M.1.1  "D042044"    
D10.D042044.3_9_M.1.1 "D042044"    
E13.D042044.3_9_M.1.1 "D042044"    
F19.D042044.3_9_M.1.1 "D042044"    
H2.D042044.3_9_M.1.1  "D042044"    
rnames = row.names(barcode.df)
meta.data <- merge(barcode.df, plate_metadata, by='plate.barcode', sort = F)
row.names(meta.data) <- rnames
# Sort cells by plate barcode because that's how the data was originally
meta.data = meta.data[order(meta.data$plate.barcode), ]
corner(meta.data)
raw.data = raw.data[, rownames(meta.data)]
corner(raw.data)

Process the raw data and load it into the Seurat object.

# Find ERCC's, compute the percent ERCC, and drop them from the raw data.
erccs <- grep(pattern = "^ERCC-", x = rownames(x = raw.data), value = TRUE)
percent.ercc <- Matrix::colSums(raw.data[erccs, ])/Matrix::colSums(raw.data)
ercc.index <- grep(pattern = "^ERCC-", x = rownames(x = raw.data), value = FALSE)
raw.data <- raw.data[-ercc.index,]
# Create the Seurat object with all the data
tiss <- CreateSeuratObject(raw.data = raw.data, project = tissue_of_interest, 
                    min.cells = 5, min.genes = 5)
tiss <- AddMetaData(object = tiss, meta.data)
tiss <- AddMetaData(object = tiss, percent.ercc, col.name = "percent.ercc")
# Change default name for sums of counts from nUMI to nReads
colnames(tiss@meta.data)[colnames(tiss@meta.data) == 'nUMI'] <- 'nReads'
# Create metadata columns for annotations and subannotations
tiss@meta.data[,'annotation'] <- NA
tiss@meta.data[,'subannotation'] <- NA

Calculate percent ribosomal genes.

ribo.genes <- grep(pattern = "^Rp[sl][[:digit:]]", x = rownames(x = tiss@data), value = TRUE)
percent.ribo <- Matrix::colSums(tiss@raw.data[ribo.genes, ])/Matrix::colSums(tiss@raw.data)
tiss <- AddMetaData(object = tiss, metadata = percent.ribo, col.name = "percent.ribo")

A sanity check: genes per cell vs reads per cell.

GenePlot(object = tiss, gene1 = "nReads", gene2 = "nGene", use.raw=T)

Filter out cells with few reads and few genes.

tiss <- FilterCells(object = tiss, subset.names = c("nGene", "nReads"), 
    low.thresholds = c(500, 50000), high.thresholds = c(25000, 2000000))

Normalize the data, then regress out correlation with total reads

tiss <- NormalizeData(object = tiss)
Performing log-normalization
0%   10   20   30   40   50   60   70   80   90   100%
|----|----|----|----|----|----|----|----|----|----|
**************************************************|
tiss <- ScaleData(object = tiss, vars.to.regress = c("nReads", "percent.ribo","Rn45s"))
[1] "Regressing out nReads"       "Regressing out percent.ribo" "Regressing out Rn45s"       

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[1] "Scaling data matrix"

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tiss <- FindVariableGenes(object = tiss, do.plot = TRUE, x.high.cutoff = Inf, y.cutoff = 0.5)
Calculating gene means
0%   10   20   30   40   50   60   70   80   90   100%
|----|----|----|----|----|----|----|----|----|----|
**************************************************|
Calculating gene variance to mean ratios
0%   10   20   30   40   50   60   70   80   90   100%
|----|----|----|----|----|----|----|----|----|----|
**************************************************|

Run Principal Component Analysis.

tiss <- RunPCA(object = tiss, do.print = FALSE)
tiss <- ProjectPCA(object = tiss, do.print = FALSE)

Later on (in FindClusters and TSNE) you will pick a number of principal components to use. This has the effect of keeping the major directions of variation in the data and, ideally, supressing noise. There is no correct answer to the number to use, but a decent rule of thumb is to go until the plot plateaus.

PCElbowPlot(object = tiss)

Choose the number of principal components to use.

# Set number of principal components. 
n.pcs = 15

The clustering is performed based on a nearest neighbors graph. Cells that have similar expression will be joined together. The Louvain algorithm looks for groups of cells with high modularity–more connections within the group than between groups. The resolution parameter determines the scale…higher resolution will give more clusters, lower resolution will give fewer.

For the top-level clustering, aim to under-cluster instead of over-cluster. It will be easy to subset groups and further analyze them below.

# Set resolution 
res.used <- 0.5
tiss <- FindClusters(object = tiss, reduction.type = "pca", dims.use = 1:n.pcs, 
    resolution = res.used, print.output = 0, save.SNN = TRUE)

To visualize

# If cells are too spread out, you can raise the perplexity. If you have few cells, try a lower perplexity (but never less than 10).
tiss <- RunTSNE(object = tiss, dims.use = 1:n.pcs, seed.use = 10, perplexity=84)
# note that you can set do.label=T to help label individual clusters
TSNEPlot(object = tiss, do.label = T)

Check expression of genes of interset.

Dotplots let you see the intensity of exppression and the fraction of cells expressing for each of your genes of interest.

How big are the clusters?

table(tiss@ident)

   0    1    2    3    4    5    6    7    8    9 
1137  863  770  700  324  285  278  210  177  153 

Which markers identify a specific cluster?

clust.markers <- FindMarkers(object = tiss, ident.1 = 0, only.pos = TRUE, min.pct = 0.25, thresh.use = 0.25)

   |                                                  | 0 % ~calculating  
   |+                                                 | 1 % ~02m 10s      
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   |+++++++++++++++++++++++++++++++++++++++++++++    | 90% ~18s          
   |++++++++++++++++++++++++++++++++++++++++++++++    | 91% ~16s          
   |++++++++++++++++++++++++++++++++++++++++++++++   | 92% ~14s          
   |+++++++++++++++++++++++++++++++++++++++++++++++   | 93% ~12s          
   |+++++++++++++++++++++++++++++++++++++++++++++++   | 94% ~11s          
   |++++++++++++++++++++++++++++++++++++++++++++++++  | 95% ~09s          
   |++++++++++++++++++++++++++++++++++++++++++++++++  | 96% ~07s          
   |+++++++++++++++++++++++++++++++++++++++++++++++++ | 97% ~05s          
   |+++++++++++++++++++++++++++++++++++++++++++++++++ | 98% ~04s          
   |++++++++++++++++++++++++++++++++++++++++++++++++++| 99% ~02s          
   |++++++++++++++++++++++++++++++++++++++++++++++++++| 100% elapsed = 02m 55s
print(x = head(x= clust.markers, n = 10))

You can also compute all markers for all clusters at once. This may take some time.

tiss.markers <- FindAllMarkers(object = tiss, only.pos = TRUE, min.pct = 0.25, thresh.use = 0.25)

   |                                                  | 0 % ~calculating  
   |+                                                 | 1 % ~03m 03s      
   |++                                                | 2 % ~03m 08s      
   |++                                                | 3 % ~03m 06s      
   |+++                                               | 4 % ~03m 00s      
   |+++                                               | 5 % ~02m 55s      
   |++++                                              | 6 % ~02m 57s      
   |++++                                              | 7 % ~02m 57s      
   |+++++                                             | 8 % ~02m 59s      
   |+++++                                             | 9 % ~03m 01s      
   |++++++                                            | 11% ~03m 04s      
   |++++++                                            | 12% ~03m 02s      
   |+++++++                                           | 13% ~02m 58s      
   |+++++++                                           | 14% ~02m 54s      
   |++++++++                                          | 15% ~02m 49s      
   |++++++++                                          | 16% ~02m 46s      
   |+++++++++                                         | 17% ~02m 43s      
   |+++++++++                                         | 18% ~02m 39s      
   |++++++++++                                        | 19% ~02m 36s      
   |++++++++++                                        | 20% ~02m 33s      
   |+++++++++++                                       | 21% ~02m 31s      
   |++++++++++++                                      | 22% ~02m 29s      
   |++++++++++++                                      | 23% ~02m 26s      
   |+++++++++++++                                     | 24% ~02m 23s      
   |+++++++++++++                                     | 25% ~02m 21s      
   |++++++++++++++                                    | 26% ~02m 19s      
   |++++++++++++++                                    | 27% ~02m 17s      
   |+++++++++++++++                                   | 28% ~02m 15s      
   |+++++++++++++++                                   | 29% ~02m 14s      
   |++++++++++++++++                                  | 31% ~02m 13s      
   |++++++++++++++++                                  | 32% ~02m 12s      
   |+++++++++++++++++                                 | 33% ~02m 10s      
   |+++++++++++++++++                                 | 34% ~02m 07s      
   |++++++++++++++++++                                | 35% ~02m 05s      
   |++++++++++++++++++                                | 36% ~02m 02s      
   |+++++++++++++++++++                               | 37% ~02m 00s      
   |+++++++++++++++++++                               | 38% ~01m 58s      
   |++++++++++++++++++++                              | 39% ~01m 55s      
   |++++++++++++++++++++                              | 40% ~01m 53s      
   |+++++++++++++++++++++                             | 41% ~01m 51s      
   |++++++++++++++++++++++                            | 42% ~01m 49s      
   |++++++++++++++++++++++                            | 43% ~01m 48s      
   |+++++++++++++++++++++++                           | 44% ~01m 46s      
   |+++++++++++++++++++++++                           | 45% ~01m 44s      
   |++++++++++++++++++++++++                          | 46% ~01m 42s      
   |++++++++++++++++++++++++                          | 47% ~01m 40s      
   |+++++++++++++++++++++++++                         | 48% ~01m 37s      
   |+++++++++++++++++++++++++                         | 49% ~01m 35s      
   |++++++++++++++++++++++++++                        | 51% ~01m 33s      
   |++++++++++++++++++++++++++                        | 52% ~01m 31s      
   |+++++++++++++++++++++++++++                       | 53% ~01m 29s      
   |+++++++++++++++++++++++++++                       | 54% ~01m 27s      
   |++++++++++++++++++++++++++++                      | 55% ~01m 24s      
   |++++++++++++++++++++++++++++                      | 56% ~01m 22s      
   |+++++++++++++++++++++++++++++                     | 57% ~01m 20s      
   |+++++++++++++++++++++++++++++                     | 58% ~01m 18s      
   |++++++++++++++++++++++++++++++                    | 59% ~01m 16s      
   |++++++++++++++++++++++++++++++                    | 60% ~01m 14s      
   |+++++++++++++++++++++++++++++++                   | 61% ~01m 12s      
   |++++++++++++++++++++++++++++++++                  | 62% ~01m 10s      
   |++++++++++++++++++++++++++++++++                  | 63% ~01m 08s      
   |+++++++++++++++++++++++++++++++++                 | 64% ~01m 06s      
   |+++++++++++++++++++++++++++++++++                 | 65% ~01m 04s      
   |++++++++++++++++++++++++++++++++++                | 66% ~01m 02s      
   |++++++++++++++++++++++++++++++++++                | 67% ~60s          
   |+++++++++++++++++++++++++++++++++++               | 68% ~58s          
   |+++++++++++++++++++++++++++++++++++               | 69% ~56s          
   |++++++++++++++++++++++++++++++++++++              | 71% ~54s          
   |++++++++++++++++++++++++++++++++++++              | 72% ~52s          
   |+++++++++++++++++++++++++++++++++++++             | 73% ~50s          
   |+++++++++++++++++++++++++++++++++++++             | 74% ~48s          
   |++++++++++++++++++++++++++++++++++++++            | 75% ~46s          
   |++++++++++++++++++++++++++++++++++++++            | 76% ~44s          
   |+++++++++++++++++++++++++++++++++++++++           | 77% ~42s          
   |+++++++++++++++++++++++++++++++++++++++           | 78% ~40s          
   |++++++++++++++++++++++++++++++++++++++++          | 79% ~38s          
   |++++++++++++++++++++++++++++++++++++++++         | 80% ~36s          
   |+++++++++++++++++++++++++++++++++++++++++         | 81% ~34s          
   |++++++++++++++++++++++++++++++++++++++++++        | 82% ~32s          
   |++++++++++++++++++++++++++++++++++++++++++        | 83% ~30s          
   |+++++++++++++++++++++++++++++++++++++++++++       | 84% ~28s          
   |+++++++++++++++++++++++++++++++++++++++++++       | 85% ~26s          
   |++++++++++++++++++++++++++++++++++++++++++++      | 86% ~24s          
   |++++++++++++++++++++++++++++++++++++++++++++      | 87% ~22s          
   |+++++++++++++++++++++++++++++++++++++++++++++     | 88% ~21s          
   |+++++++++++++++++++++++++++++++++++++++++++++     | 89% ~19s          
   |++++++++++++++++++++++++++++++++++++++++++++++    | 91% ~17s          
   |++++++++++++++++++++++++++++++++++++++++++++++    | 92% ~15s          
   |+++++++++++++++++++++++++++++++++++++++++++++++   | 93% ~13s          
   |+++++++++++++++++++++++++++++++++++++++++++++++   | 94% ~11s          
   |++++++++++++++++++++++++++++++++++++++++++++++++  | 95% ~09s          
   |++++++++++++++++++++++++++++++++++++++++++++++++  | 96% ~07s          
   |+++++++++++++++++++++++++++++++++++++++++++++++++ | 97% ~05s          
   |+++++++++++++++++++++++++++++++++++++++++++++++++ | 98% ~04s          
   |++++++++++++++++++++++++++++++++++++++++++++++++++| 99% ~02s          
   |++++++++++++++++++++++++++++++++++++++++++++++++++| 100% elapsed = 02m 53s

   |                                                  | 0 % ~calculating  
   |+                                                 | 1 % ~01m 32s      
   |++                                                | 2 % ~01m 37s      
   |++                                                | 3 % ~01m 40s      
   |+++                                               | 4 % ~01m 33s      
   |+++                                               | 5 % ~01m 29s      
   |++++                                              | 6 % ~01m 26s      
   |++++                                              | 7 % ~01m 24s      
   |+++++                                             | 8 % ~01m 22s      
   |+++++                                             | 9 % ~01m 20s      
   |++++++                                            | 10% ~01m 18s      
   |++++++                                            | 11% ~01m 17s      
   |+++++++                                           | 12% ~01m 15s      
   |+++++++                                           | 13% ~01m 15s      
   |++++++++                                          | 14% ~01m 14s      
   |++++++++                                          | 15% ~01m 13s      
   |+++++++++                                         | 16% ~01m 12s      
   |+++++++++                                         | 18% ~01m 11s      
   |++++++++++                                        | 19% ~01m 09s      
   |++++++++++                                        | 20% ~01m 08s      
   |+++++++++++                                       | 21% ~01m 07s      
   |+++++++++++                                       | 22% ~01m 06s      
   |++++++++++++                                      | 23% ~01m 05s      
   |++++++++++++                                      | 24% ~01m 04s      
   |+++++++++++++                                     | 25% ~01m 03s      
   |+++++++++++++                                     | 26% ~01m 03s      
   |++++++++++++++                                    | 27% ~01m 02s      
   |++++++++++++++                                    | 28% ~01m 01s      
   |+++++++++++++++                                   | 29% ~01m 00s      
   |+++++++++++++++                                   | 30% ~59s          
   |++++++++++++++++                                  | 31% ~58s          
   |++++++++++++++++                                  | 32% ~57s          
   |+++++++++++++++++                                 | 33% ~56s          
   |++++++++++++++++++                                | 34% ~55s          
   |++++++++++++++++++                                | 35% ~54s          
   |+++++++++++++++++++                               | 36% ~53s          
   |+++++++++++++++++++                               | 37% ~52s          
   |++++++++++++++++++++                              | 38% ~52s          
   |++++++++++++++++++++                              | 39% ~51s          
   |+++++++++++++++++++++                             | 40% ~50s          
   |+++++++++++++++++++++                             | 41% ~49s          
   |++++++++++++++++++++++                            | 42% ~48s          
   |++++++++++++++++++++++                            | 43% ~47s          
   |+++++++++++++++++++++++                           | 44% ~46s          
   |+++++++++++++++++++++++                           | 45% ~45s          
   |++++++++++++++++++++++++                          | 46% ~45s          
   |++++++++++++++++++++++++                          | 47% ~44s          
   |+++++++++++++++++++++++++                         | 48% ~43s          
   |+++++++++++++++++++++++++                         | 49% ~42s          
   |++++++++++++++++++++++++++                        | 51% ~41s          
   |++++++++++++++++++++++++++                        | 52% ~40s          
   |+++++++++++++++++++++++++++                       | 53% ~39s          
   |+++++++++++++++++++++++++++                       | 54% ~38s          
   |++++++++++++++++++++++++++++                      | 55% ~38s          
   |++++++++++++++++++++++++++++                      | 56% ~37s          
   |+++++++++++++++++++++++++++++                     | 57% ~36s          
   |+++++++++++++++++++++++++++++                     | 58% ~35s          
   |++++++++++++++++++++++++++++++                    | 59% ~34s          
   |++++++++++++++++++++++++++++++                    | 60% ~33s          
   |+++++++++++++++++++++++++++++++                   | 61% ~32s          
   |+++++++++++++++++++++++++++++++                   | 62% ~32s          
   |++++++++++++++++++++++++++++++++                  | 63% ~31s          
   |++++++++++++++++++++++++++++++++                  | 64% ~30s          
   |+++++++++++++++++++++++++++++++++                 | 65% ~29s          
   |+++++++++++++++++++++++++++++++++                 | 66% ~28s          
   |++++++++++++++++++++++++++++++++++                | 67% ~27s          
   |+++++++++++++++++++++++++++++++++++               | 68% ~26s          
   |+++++++++++++++++++++++++++++++++++               | 69% ~26s          
   |++++++++++++++++++++++++++++++++++++              | 70% ~25s          
   |++++++++++++++++++++++++++++++++++++              | 71% ~24s          
   |+++++++++++++++++++++++++++++++++++++             | 72% ~23s          
   |+++++++++++++++++++++++++++++++++++++             | 73% ~22s          
   |++++++++++++++++++++++++++++++++++++++            | 74% ~21s          
   |++++++++++++++++++++++++++++++++++++++            | 75% ~21s          
   |+++++++++++++++++++++++++++++++++++++++           | 76% ~20s          
   |+++++++++++++++++++++++++++++++++++++++           | 77% ~19s          
   |++++++++++++++++++++++++++++++++++++++++          | 78% ~18s          
   |++++++++++++++++++++++++++++++++++++++++          | 79% ~17s          
   |+++++++++++++++++++++++++++++++++++++++++         | 80% ~16s          
   |+++++++++++++++++++++++++++++++++++++++++         | 81% ~15s          
   |++++++++++++++++++++++++++++++++++++++++++        | 82% ~15s          
   |++++++++++++++++++++++++++++++++++++++++++        | 84% ~14s          
   |+++++++++++++++++++++++++++++++++++++++++++       | 85% ~13s          
   |+++++++++++++++++++++++++++++++++++++++++++       | 86% ~12s          
   |++++++++++++++++++++++++++++++++++++++++++++      | 87% ~11s          
   |++++++++++++++++++++++++++++++++++++++++++++      | 88% ~10s          
   |+++++++++++++++++++++++++++++++++++++++++++++     | 89% ~10s          
   |+++++++++++++++++++++++++++++++++++++++++++++     | 90% ~09s          
   |++++++++++++++++++++++++++++++++++++++++++++++    | 91% ~08s          
   |++++++++++++++++++++++++++++++++++++++++++++++    | 92% ~07s          
   |+++++++++++++++++++++++++++++++++++++++++++++++   | 93% ~06s          
   |+++++++++++++++++++++++++++++++++++++++++++++++   | 94% ~05s          
   |++++++++++++++++++++++++++++++++++++++++++++++++  | 95% ~04s          
   |++++++++++++++++++++++++++++++++++++++++++++++++  | 96% ~03s          
   |+++++++++++++++++++++++++++++++++++++++++++++++++ | 97% ~03s          
   |+++++++++++++++++++++++++++++++++++++++++++++++++ | 98% ~02s          
   |++++++++++++++++++++++++++++++++++++++++++++++++++| 99% ~01s          
   |++++++++++++++++++++++++++++++++++++++++++++++++++| 100% elapsed = 01m 24s

   |                                                  | 0 % ~calculating  
   |+                                                 | 1 % ~05m 10s      
   |++                                                | 2 % ~05m 24s      
   |++                                                | 3 % ~05m 08s      
   |+++                                               | 4 % ~05m 14s      
   |+++                                               | 5 % ~05m 09s      
   |++++                                              | 6 % ~04m 60s      
   |++++                                              | 7 % ~04m 50s      
   |+++++                                             | 8 % ~04m 46s      
   |+++++                                             | 9 % ~04m 38s      
   |++++++                                            | 10% ~04m 31s      
   |++++++                                            | 11% ~04m 24s      
   |+++++++                                           | 12% ~04m 19s      
   |+++++++                                           | 13% ~04m 14s      
   |++++++++                                          | 14% ~04m 09s      
   |++++++++                                          | 15% ~04m 05s      
   |+++++++++                                         | 16% ~04m 01s      
   |+++++++++                                         | 17% ~03m 56s      
   |++++++++++                                        | 18% ~03m 52s      
   |++++++++++                                        | 19% ~03m 49s      
   |+++++++++++                                       | 20% ~03m 45s      
   |+++++++++++                                       | 21% ~03m 42s      
   |++++++++++++                                      | 22% ~03m 38s      
   |++++++++++++                                      | 23% ~03m 36s      
   |+++++++++++++                                     | 24% ~03m 32s      
   |+++++++++++++                                     | 25% ~03m 30s      
   |++++++++++++++                                    | 26% ~03m 28s      
   |++++++++++++++                                    | 27% ~03m 25s      
   |+++++++++++++++                                   | 28% ~03m 23s      
   |+++++++++++++++                                   | 29% ~03m 20s      
   |++++++++++++++++                                  | 30% ~03m 17s      
   |++++++++++++++++                                  | 31% ~03m 14s      
   |+++++++++++++++++                                 | 32% ~03m 12s      
   |+++++++++++++++++                                 | 33% ~03m 09s      
   |++++++++++++++++++                                | 34% ~03m 06s      
   |++++++++++++++++++                                | 35% ~03m 04s      
   |+++++++++++++++++++                               | 36% ~03m 01s      
   |+++++++++++++++++++                               | 37% ~02m 58s      
   |++++++++++++++++++++                              | 38% ~02m 56s      
   |++++++++++++++++++++                              | 39% ~02m 53s      
   |+++++++++++++++++++++                             | 40% ~02m 50s      
   |+++++++++++++++++++++                             | 41% ~02m 47s      
   |++++++++++++++++++++++                            | 42% ~02m 44s      
   |++++++++++++++++++++++                            | 43% ~02m 41s      
   |+++++++++++++++++++++++                           | 44% ~02m 38s      
   |+++++++++++++++++++++++                           | 45% ~02m 36s      
   |++++++++++++++++++++++++                          | 46% ~02m 33s      
   |++++++++++++++++++++++++                          | 47% ~02m 30s      
   |+++++++++++++++++++++++++                         | 48% ~02m 27s      
   |+++++++++++++++++++++++++                         | 49% ~02m 24s      
   |++++++++++++++++++++++++++                        | 51% ~02m 21s      
   |++++++++++++++++++++++++++                        | 52% ~02m 18s      
   |+++++++++++++++++++++++++++                       | 53% ~02m 15s      
   |+++++++++++++++++++++++++++                       | 54% ~02m 12s      
   |++++++++++++++++++++++++++++                      | 55% ~02m 10s      
   |++++++++++++++++++++++++++++                      | 56% ~02m 07s      
   |+++++++++++++++++++++++++++++                     | 57% ~02m 04s      
   |+++++++++++++++++++++++++++++                     | 58% ~02m 01s      
   |++++++++++++++++++++++++++++++                    | 59% ~01m 58s      
   |++++++++++++++++++++++++++++++                    | 60% ~01m 55s      
   |+++++++++++++++++++++++++++++++                   | 61% ~01m 52s      
   |+++++++++++++++++++++++++++++++                   | 62% ~01m 49s      
   |++++++++++++++++++++++++++++++++                  | 63% ~01m 46s      
   |++++++++++++++++++++++++++++++++                  | 64% ~01m 42s      
   |+++++++++++++++++++++++++++++++++                 | 65% ~01m 39s      
   |+++++++++++++++++++++++++++++++++                 | 66% ~01m 37s      
   |++++++++++++++++++++++++++++++++++                | 67% ~01m 34s      
   |++++++++++++++++++++++++++++++++++                | 68% ~01m 31s      
   |+++++++++++++++++++++++++++++++++++               | 69% ~01m 28s      
   |+++++++++++++++++++++++++++++++++++               | 70% ~01m 25s      
   |++++++++++++++++++++++++++++++++++++              | 71% ~01m 22s      
   |++++++++++++++++++++++++++++++++++++              | 72% ~01m 19s      
   |+++++++++++++++++++++++++++++++++++++             | 73% ~01m 16s      
   |+++++++++++++++++++++++++++++++++++++             | 74% ~01m 13s      
   |++++++++++++++++++++++++++++++++++++++            | 75% ~01m 10s      
   |++++++++++++++++++++++++++++++++++++++            | 76% ~01m 07s      
   |+++++++++++++++++++++++++++++++++++++++           | 77% ~01m 05s      
   |+++++++++++++++++++++++++++++++++++++++           | 78% ~01m 02s      
   |++++++++++++++++++++++++++++++++++++++++          | 79% ~59s          
   |++++++++++++++++++++++++++++++++++++++++          | 80% ~56s          
   |+++++++++++++++++++++++++++++++++++++++++         | 81% ~53s          
   |+++++++++++++++++++++++++++++++++++++++++         | 82% ~50s          
   |++++++++++++++++++++++++++++++++++++++++++        | 83% ~47s          
   |++++++++++++++++++++++++++++++++++++++++++        | 84% ~44s          
   |+++++++++++++++++++++++++++++++++++++++++++       | 85% ~42s          
   |+++++++++++++++++++++++++++++++++++++++++++       | 86% ~39s          
   |++++++++++++++++++++++++++++++++++++++++++++      | 87% ~36s          
   |++++++++++++++++++++++++++++++++++++++++++++      | 88% ~33s          
   |+++++++++++++++++++++++++++++++++++++++++++++     | 89% ~30s          
   |+++++++++++++++++++++++++++++++++++++++++++++     | 90% ~27s          
   |++++++++++++++++++++++++++++++++++++++++++++++    | 91% ~25s          
   |++++++++++++++++++++++++++++++++++++++++++++++    | 92% ~22s          
   |+++++++++++++++++++++++++++++++++++++++++++++++   | 93% ~19s          
   |+++++++++++++++++++++++++++++++++++++++++++++++   | 94% ~16s          
   |++++++++++++++++++++++++++++++++++++++++++++++++  | 95% ~14s          
   |++++++++++++++++++++++++++++++++++++++++++++++++  | 96% ~11s          
   |+++++++++++++++++++++++++++++++++++++++++++++++++ | 97% ~08s          
   |+++++++++++++++++++++++++++++++++++++++++++++++++ | 98% ~05s          
   |++++++++++++++++++++++++++++++++++++++++++++++++++| 99% ~03s          
   |++++++++++++++++++++++++++++++++++++++++++++++++++| 100% elapsed = 04m 28s

   |                                                  | 0 % ~calculating  
   |+                                                 | 1 % ~01m 07s      
   |++                                                | 2 % ~01m 07s      
   |++                                                | 3 % ~01m 06s      
   |+++                                               | 4 % ~01m 05s      
   |+++                                               | 5 % ~01m 05s      
   |++++                                              | 6 % ~01m 04s      
   |++++                                              | 8 % ~01m 04s      
   |+++++                                             | 9 % ~01m 03s      
   |+++++                                             | 10% ~01m 03s      
   |++++++                                            | 11% ~01m 02s      
   |++++++                                            | 12% ~01m 02s      
   |+++++++                                           | 13% ~01m 01s      
   |+++++++                                           | 14% ~01m 01s      
   |++++++++                                          | 15% ~01m 00s      
   |+++++++++                                         | 16% ~59s          
   |+++++++++                                         | 17% ~59s          
   |++++++++++                                        | 18% ~58s          
   |++++++++++                                        | 19% ~57s          
   |+++++++++++                                       | 20% ~56s          
   |+++++++++++                                       | 22% ~56s          
   |++++++++++++                                      | 23% ~55s          
   |++++++++++++                                      | 24% ~54s          
   |+++++++++++++                                     | 25% ~53s          
   |+++++++++++++                                     | 26% ~52s          
   |++++++++++++++                                    | 27% ~51s          
   |++++++++++++++                                    | 28% ~50s          
   |+++++++++++++++                                   | 29% ~50s          
   |++++++++++++++++                                  | 30% ~49s          
   |++++++++++++++++                                  | 31% ~48s          
   |+++++++++++++++++                                 | 32% ~47s          
   |+++++++++++++++++                                 | 33% ~47s          
   |++++++++++++++++++                                | 34% ~46s          
   |++++++++++++++++++                                | 35% ~45s          
   |+++++++++++++++++++                               | 37% ~44s          
   |+++++++++++++++++++                               | 38% ~43s          
   |++++++++++++++++++++                              | 39% ~42s          
   |++++++++++++++++++++                              | 40% ~42s          
   |+++++++++++++++++++++                             | 41% ~41s          
   |+++++++++++++++++++++                             | 42% ~40s          
   |++++++++++++++++++++++                            | 43% ~39s          
   |+++++++++++++++++++++++                           | 44% ~39s          
   |+++++++++++++++++++++++                           | 45% ~38s          
   |++++++++++++++++++++++++                          | 46% ~37s          
   |++++++++++++++++++++++++                          | 47% ~36s          
   |+++++++++++++++++++++++++                         | 48% ~35s          
   |+++++++++++++++++++++++++                         | 49% ~35s          
   |++++++++++++++++++++++++++                        | 51% ~34s          
   |++++++++++++++++++++++++++                        | 52% ~33s          
   |+++++++++++++++++++++++++++                       | 53% ~32s          
   |+++++++++++++++++++++++++++                       | 54% ~32s          
   |++++++++++++++++++++++++++++                      | 55% ~31s          
   |++++++++++++++++++++++++++++                      | 56% ~30s          
   |+++++++++++++++++++++++++++++                     | 57% ~30s          
   |++++++++++++++++++++++++++++++                    | 58% ~29s          
   |++++++++++++++++++++++++++++++                    | 59% ~28s          
   |+++++++++++++++++++++++++++++++                   | 60% ~27s          
   |+++++++++++++++++++++++++++++++                   | 61% ~26s          
   |++++++++++++++++++++++++++++++++                  | 62% ~26s          
   |++++++++++++++++++++++++++++++++                  | 63% ~25s          
   |+++++++++++++++++++++++++++++++++                 | 65% ~24s          
   |+++++++++++++++++++++++++++++++++                 | 66% ~23s          
   |++++++++++++++++++++++++++++++++++                | 67% ~23s          
   |++++++++++++++++++++++++++++++++++                | 68% ~22s          
   |+++++++++++++++++++++++++++++++++++               | 69% ~21s          
   |+++++++++++++++++++++++++++++++++++               | 70% ~20s          
   |++++++++++++++++++++++++++++++++++++              | 71% ~20s          
   |+++++++++++++++++++++++++++++++++++++             | 72% ~19s          
   |+++++++++++++++++++++++++++++++++++++             | 73% ~18s          
   |++++++++++++++++++++++++++++++++++++++            | 74% ~18s          
   |++++++++++++++++++++++++++++++++++++++            | 75% ~17s          
   |+++++++++++++++++++++++++++++++++++++++           | 76% ~16s          
   |+++++++++++++++++++++++++++++++++++++++           | 77% ~15s          
   |++++++++++++++++++++++++++++++++++++++++          | 78% ~15s          
   |++++++++++++++++++++++++++++++++++++++++          | 80% ~14s          
   |+++++++++++++++++++++++++++++++++++++++++         | 81% ~13s          
   |+++++++++++++++++++++++++++++++++++++++++         | 82% ~12s          
   |++++++++++++++++++++++++++++++++++++++++++        | 83% ~12s          
   |++++++++++++++++++++++++++++++++++++++++++        | 84% ~11s          
   |+++++++++++++++++++++++++++++++++++++++++++       | 85% ~10s          
   |++++++++++++++++++++++++++++++++++++++++++++      | 86% ~09s          
   |++++++++++++++++++++++++++++++++++++++++++++      | 87% ~09s          
   |+++++++++++++++++++++++++++++++++++++++++++++     | 88% ~08s          
   |+++++++++++++++++++++++++++++++++++++++++++++     | 89% ~07s          
   |++++++++++++++++++++++++++++++++++++++++++++++    | 90% ~07s          
   |++++++++++++++++++++++++++++++++++++++++++++++    | 91% ~06s          
   |+++++++++++++++++++++++++++++++++++++++++++++++   | 92% ~05s          
   |+++++++++++++++++++++++++++++++++++++++++++++++   | 94% ~04s          
   |++++++++++++++++++++++++++++++++++++++++++++++++  | 95% ~04s          
   |++++++++++++++++++++++++++++++++++++++++++++++++  | 96% ~03s          
   |+++++++++++++++++++++++++++++++++++++++++++++++++ | 97% ~02s          
   |+++++++++++++++++++++++++++++++++++++++++++++++++ | 98% ~01s          
   |++++++++++++++++++++++++++++++++++++++++++++++++++| 99% ~01s          
   |++++++++++++++++++++++++++++++++++++++++++++++++++| 100% elapsed = 01m 07s

   |                                                  | 0 % ~calculating  
   |+                                                 | 1 % ~01m 05s      
   |++                                                | 2 % ~01m 04s      
   |++                                                | 3 % ~01m 04s      
   |+++                                               | 4 % ~01m 03s      
   |+++                                               | 5 % ~01m 02s      
   |++++                                              | 6 % ~01m 02s      
   |++++                                              | 7 % ~01m 01s      
   |+++++                                             | 8 % ~01m 01s      
   |+++++                                             | 9 % ~60s          
   |++++++                                            | 11% ~59s          
   |++++++                                            | 12% ~58s          
   |+++++++                                           | 13% ~58s          
   |+++++++                                           | 14% ~57s          
   |++++++++                                          | 15% ~56s          
   |++++++++                                          | 16% ~56s          
   |+++++++++                                         | 17% ~55s          
   |+++++++++                                         | 18% ~54s          
   |++++++++++                                        | 19% ~53s          
   |++++++++++                                        | 20% ~53s          
   |+++++++++++                                       | 21% ~52s          
   |++++++++++++                                      | 22% ~51s          
   |++++++++++++                                      | 23% ~51s          
   |+++++++++++++                                     | 24% ~50s          
   |+++++++++++++                                     | 25% ~49s          
   |++++++++++++++                                    | 26% ~49s          
   |++++++++++++++                                    | 27% ~48s          
   |+++++++++++++++                                   | 28% ~47s          
   |+++++++++++++++                                   | 29% ~47s          
   |++++++++++++++++                                  | 31% ~46s          
   |++++++++++++++++                                  | 32% ~45s          
   |+++++++++++++++++                                 | 33% ~45s          
   |+++++++++++++++++                                 | 34% ~44s          
   |++++++++++++++++++                                | 35% ~43s          
   |++++++++++++++++++                                | 36% ~42s          
   |+++++++++++++++++++                               | 37% ~42s          
   |+++++++++++++++++++                               | 38% ~41s          
   |++++++++++++++++++++                              | 39% ~40s          
   |++++++++++++++++++++                              | 40% ~40s          
   |+++++++++++++++++++++                             | 41% ~39s          
   |++++++++++++++++++++++                            | 42% ~38s          
   |++++++++++++++++++++++                            | 43% ~38s          
   |+++++++++++++++++++++++                           | 44% ~37s          
   |+++++++++++++++++++++++                           | 45% ~36s          
   |++++++++++++++++++++++++                          | 46% ~36s          
   |++++++++++++++++++++++++                          | 47% ~35s          
   |+++++++++++++++++++++++++                         | 48% ~34s          
   |+++++++++++++++++++++++++                         | 49% ~34s          
   |++++++++++++++++++++++++++                        | 51% ~33s          
   |++++++++++++++++++++++++++                        | 52% ~32s          
   |+++++++++++++++++++++++++++                       | 53% ~31s          
   |+++++++++++++++++++++++++++                       | 54% ~31s          
   |++++++++++++++++++++++++++++                      | 55% ~30s          
   |++++++++++++++++++++++++++++                      | 56% ~29s          
   |+++++++++++++++++++++++++++++                     | 57% ~29s          
   |+++++++++++++++++++++++++++++                     | 58% ~28s          
   |++++++++++++++++++++++++++++++                    | 59% ~27s          
   |++++++++++++++++++++++++++++++                    | 60% ~27s          
   |+++++++++++++++++++++++++++++++                   | 61% ~26s          
   |++++++++++++++++++++++++++++++++                  | 62% ~25s          
   |++++++++++++++++++++++++++++++++                  | 63% ~24s          
   |+++++++++++++++++++++++++++++++++                 | 64% ~24s          
   |+++++++++++++++++++++++++++++++++                 | 65% ~23s          
   |++++++++++++++++++++++++++++++++++                | 66% ~22s          
   |++++++++++++++++++++++++++++++++++                | 67% ~22s          
   |+++++++++++++++++++++++++++++++++++               | 68% ~21s          
   |+++++++++++++++++++++++++++++++++++               | 69% ~20s          
   |++++++++++++++++++++++++++++++++++++              | 71% ~20s          
   |++++++++++++++++++++++++++++++++++++              | 72% ~19s          
   |+++++++++++++++++++++++++++++++++++++             | 73% ~18s          
   |+++++++++++++++++++++++++++++++++++++             | 74% ~18s          
   |++++++++++++++++++++++++++++++++++++++            | 75% ~17s          
   |++++++++++++++++++++++++++++++++++++++            | 76% ~16s          
   |+++++++++++++++++++++++++++++++++++++++           | 77% ~15s          
   |+++++++++++++++++++++++++++++++++++++++           | 78% ~15s          
   |++++++++++++++++++++++++++++++++++++++++          | 79% ~14s          
   |++++++++++++++++++++++++++++++++++++++++         | 80% ~13s          
   |+++++++++++++++++++++++++++++++++++++++++         | 81% ~13s          
   |++++++++++++++++++++++++++++++++++++++++++        | 82% ~12s          
   |++++++++++++++++++++++++++++++++++++++++++        | 83% ~11s          
   |+++++++++++++++++++++++++++++++++++++++++++       | 84% ~11s          
   |+++++++++++++++++++++++++++++++++++++++++++       | 85% ~10s          
   |++++++++++++++++++++++++++++++++++++++++++++      | 86% ~09s          
   |++++++++++++++++++++++++++++++++++++++++++++      | 87% ~08s          
   |+++++++++++++++++++++++++++++++++++++++++++++     | 88% ~08s          
   |+++++++++++++++++++++++++++++++++++++++++++++     | 89% ~07s          
   |++++++++++++++++++++++++++++++++++++++++++++++    | 91% ~06s          
   |++++++++++++++++++++++++++++++++++++++++++++++    | 92% ~06s          
   |+++++++++++++++++++++++++++++++++++++++++++++++   | 93% ~05s          
   |+++++++++++++++++++++++++++++++++++++++++++++++   | 94% ~04s          
   |++++++++++++++++++++++++++++++++++++++++++++++++  | 95% ~04s          
   |++++++++++++++++++++++++++++++++++++++++++++++++  | 96% ~03s          
   |+++++++++++++++++++++++++++++++++++++++++++++++++ | 97% ~02s          
   |+++++++++++++++++++++++++++++++++++++++++++++++++ | 98% ~01s          
   |++++++++++++++++++++++++++++++++++++++++++++++++++| 99% ~01s          
   |++++++++++++++++++++++++++++++++++++++++++++++++++| 100% elapsed = 01m 06s

   |                                                  | 0 % ~calculating  
   |+                                                 | 1 % ~01m 35s      
   |++                                                | 2 % ~01m 33s      
   |++                                                | 3 % ~01m 33s      
   |+++                                               | 4 % ~01m 32s      
   |+++                                               | 5 % ~01m 30s      
   |++++                                              | 6 % ~01m 30s      
   |++++                                              | 7 % ~01m 29s      
   |+++++                                             | 8 % ~01m 28s      
   |+++++                                             | 9 % ~01m 27s      
   |++++++                                            | 11% ~01m 26s      
   |++++++                                            | 12% ~01m 25s      
   |+++++++                                           | 13% ~01m 24s      
   |+++++++                                           | 14% ~01m 23s      
   |++++++++                                          | 15% ~01m 23s      
   |++++++++                                          | 16% ~01m 22s      
   |+++++++++                                         | 17% ~01m 21s      
   |+++++++++                                         | 18% ~01m 20s      
   |++++++++++                                        | 19% ~01m 19s      
   |++++++++++                                        | 20% ~01m 18s      
   |+++++++++++                                       | 21% ~01m 17s      
   |++++++++++++                                      | 22% ~01m 16s      
   |++++++++++++                                      | 23% ~01m 14s      
   |+++++++++++++                                     | 24% ~01m 13s      
   |+++++++++++++                                     | 25% ~01m 12s      
   |++++++++++++++                                    | 26% ~01m 11s      
   |++++++++++++++                                    | 27% ~01m 10s      
   |+++++++++++++++                                   | 28% ~01m 09s      
   |+++++++++++++++                                   | 29% ~01m 09s      
   |++++++++++++++++                                  | 31% ~01m 08s      
   |++++++++++++++++                                  | 32% ~01m 07s      
   |+++++++++++++++++                                 | 33% ~01m 06s      
   |+++++++++++++++++                                 | 34% ~01m 05s      
   |++++++++++++++++++                                | 35% ~01m 03s      
   |++++++++++++++++++                                | 36% ~01m 02s      
   |+++++++++++++++++++                               | 37% ~01m 01s      
   |+++++++++++++++++++                               | 38% ~01m 00s      
   |++++++++++++++++++++                              | 39% ~59s          
   |++++++++++++++++++++                              | 40% ~58s          
   |+++++++++++++++++++++                             | 41% ~57s          
   |++++++++++++++++++++++                            | 42% ~56s          
   |++++++++++++++++++++++                            | 43% ~55s          
   |+++++++++++++++++++++++                           | 44% ~54s          
   |+++++++++++++++++++++++                           | 45% ~53s          
   |++++++++++++++++++++++++                          | 46% ~52s          
   |++++++++++++++++++++++++                          | 47% ~51s          
   |+++++++++++++++++++++++++                         | 48% ~50s          
   |+++++++++++++++++++++++++                         | 49% ~49s          
   |++++++++++++++++++++++++++                        | 51% ~48s          
   |++++++++++++++++++++++++++                        | 52% ~47s          
   |+++++++++++++++++++++++++++                       | 53% ~46s          
   |+++++++++++++++++++++++++++                       | 54% ~45s          
   |++++++++++++++++++++++++++++                      | 55% ~44s          
   |++++++++++++++++++++++++++++                      | 56% ~43s          
   |+++++++++++++++++++++++++++++                     | 57% ~42s          
   |+++++++++++++++++++++++++++++                     | 58% ~41s          
   |++++++++++++++++++++++++++++++                    | 59% ~40s          
   |++++++++++++++++++++++++++++++                    | 60% ~39s          
   |+++++++++++++++++++++++++++++++                   | 61% ~38s          
   |++++++++++++++++++++++++++++++++                  | 62% ~37s          
   |++++++++++++++++++++++++++++++++                  | 63% ~36s          
   |+++++++++++++++++++++++++++++++++                 | 64% ~35s          
   |+++++++++++++++++++++++++++++++++                 | 65% ~34s          
   |++++++++++++++++++++++++++++++++++                | 66% ~33s          
   |++++++++++++++++++++++++++++++++++                | 67% ~32s          
   |+++++++++++++++++++++++++++++++++++               | 68% ~31s          
   |+++++++++++++++++++++++++++++++++++               | 69% ~30s          
   |++++++++++++++++++++++++++++++++++++              | 71% ~29s          
   |++++++++++++++++++++++++++++++++++++              | 72% ~28s          
   |+++++++++++++++++++++++++++++++++++++             | 73% ~27s          
   |+++++++++++++++++++++++++++++++++++++             | 74% ~26s          
   |++++++++++++++++++++++++++++++++++++++            | 75% ~25s          
   |++++++++++++++++++++++++++++++++++++++            | 76% ~24s          
   |+++++++++++++++++++++++++++++++++++++++           | 77% ~23s          
   |+++++++++++++++++++++++++++++++++++++++           | 78% ~21s          
   |++++++++++++++++++++++++++++++++++++++++          | 79% ~20s          
   |++++++++++++++++++++++++++++++++++++++++         | 80% ~19s          
   |+++++++++++++++++++++++++++++++++++++++++         | 81% ~18s          
   |++++++++++++++++++++++++++++++++++++++++++        | 82% ~17s          
   |++++++++++++++++++++++++++++++++++++++++++        | 83% ~16s          
   |+++++++++++++++++++++++++++++++++++++++++++       | 84% ~15s          
   |+++++++++++++++++++++++++++++++++++++++++++       | 85% ~14s          
   |++++++++++++++++++++++++++++++++++++++++++++      | 86% ~13s          
   |++++++++++++++++++++++++++++++++++++++++++++      | 87% ~12s          
   |+++++++++++++++++++++++++++++++++++++++++++++     | 88% ~11s          
   |+++++++++++++++++++++++++++++++++++++++++++++     | 89% ~10s          
   |++++++++++++++++++++++++++++++++++++++++++++++    | 91% ~09s          
   |++++++++++++++++++++++++++++++++++++++++++++++    | 92% ~08s          
   |+++++++++++++++++++++++++++++++++++++++++++++++   | 93% ~07s          
   |+++++++++++++++++++++++++++++++++++++++++++++++   | 94% ~06s          
   |++++++++++++++++++++++++++++++++++++++++++++++++  | 95% ~05s          
   |++++++++++++++++++++++++++++++++++++++++++++++++  | 96% ~04s          
   |+++++++++++++++++++++++++++++++++++++++++++++++++ | 97% ~03s          
   |+++++++++++++++++++++++++++++++++++++++++++++++++ | 98% ~02s          
   |++++++++++++++++++++++++++++++++++++++++++++++++++| 99% ~01s          
   |++++++++++++++++++++++++++++++++++++++++++++++++++| 100% elapsed = 01m 37s

   |                                                  | 0 % ~calculating  
   |+                                                 | 1 % ~58s          
   |++                                                | 2 % ~58s          
   |++                                                | 3 % ~58s          
   |+++                                               | 4 % ~01m 02s      
   |+++                                               | 5 % ~01m 00s      
   |++++                                              | 6 % ~59s          
   |++++                                              | 7 % ~58s          
   |+++++                                             | 8 % ~57s          
   |+++++                                             | 9 % ~57s          
   |++++++                                            | 10% ~56s          
   |++++++                                            | 11% ~56s          
   |+++++++                                           | 12% ~55s          
   |+++++++                                           | 13% ~54s          
   |++++++++                                          | 14% ~54s          
   |++++++++                                          | 15% ~53s          
   |+++++++++                                         | 16% ~53s          
   |+++++++++                                         | 18% ~52s          
   |++++++++++                                        | 19% ~51s          
   |++++++++++                                        | 20% ~51s          
   |+++++++++++                                       | 21% ~50s          
   |+++++++++++                                       | 22% ~49s          
   |++++++++++++                                      | 23% ~49s          
   |++++++++++++                                      | 24% ~48s          
   |+++++++++++++                                     | 25% ~47s          
   |+++++++++++++                                     | 26% ~47s          
   |++++++++++++++                                    | 27% ~46s          
   |++++++++++++++                                    | 28% ~45s          
   |+++++++++++++++                                   | 29% ~45s          
   |+++++++++++++++                                   | 30% ~44s          
   |++++++++++++++++                                  | 31% ~44s          
   |++++++++++++++++                                  | 32% ~43s          
   |+++++++++++++++++                                 | 33% ~42s          
   |++++++++++++++++++                                | 34% ~42s          
   |++++++++++++++++++                                | 35% ~41s          
   |+++++++++++++++++++                               | 36% ~40s          
   |+++++++++++++++++++                               | 37% ~40s          
   |++++++++++++++++++++                              | 38% ~39s          
   |++++++++++++++++++++                              | 39% ~38s          
   |+++++++++++++++++++++                             | 40% ~38s          
   |+++++++++++++++++++++                             | 41% ~37s          
   |++++++++++++++++++++++                            | 42% ~36s          
   |++++++++++++++++++++++                            | 43% ~36s          
   |+++++++++++++++++++++++                           | 44% ~35s          
   |+++++++++++++++++++++++                           | 45% ~34s          
   |++++++++++++++++++++++++                          | 46% ~34s          
   |++++++++++++++++++++++++                          | 47% ~33s          
   |+++++++++++++++++++++++++                         | 48% ~33s          
   |+++++++++++++++++++++++++                         | 49% ~32s          
   |++++++++++++++++++++++++++                        | 51% ~31s          
   |++++++++++++++++++++++++++                        | 52% ~31s          
   |+++++++++++++++++++++++++++                       | 53% ~30s          
   |+++++++++++++++++++++++++++                       | 54% ~29s          
   |++++++++++++++++++++++++++++                      | 55% ~29s          
   |++++++++++++++++++++++++++++                      | 56% ~28s          
   |+++++++++++++++++++++++++++++                     | 57% ~27s          
   |+++++++++++++++++++++++++++++                     | 58% ~27s          
   |++++++++++++++++++++++++++++++                    | 59% ~26s          
   |++++++++++++++++++++++++++++++                    | 60% ~26s          
   |+++++++++++++++++++++++++++++++                   | 61% ~25s          
   |+++++++++++++++++++++++++++++++                   | 62% ~24s          
   |++++++++++++++++++++++++++++++++                  | 63% ~24s          
   |++++++++++++++++++++++++++++++++                  | 64% ~23s          
   |+++++++++++++++++++++++++++++++++                 | 65% ~22s          
   |+++++++++++++++++++++++++++++++++                 | 66% ~22s          
   |++++++++++++++++++++++++++++++++++                | 67% ~21s          
   |+++++++++++++++++++++++++++++++++++               | 68% ~20s          
   |+++++++++++++++++++++++++++++++++++               | 69% ~20s          
   |++++++++++++++++++++++++++++++++++++              | 70% ~19s          
   |++++++++++++++++++++++++++++++++++++              | 71% ~18s          
   |+++++++++++++++++++++++++++++++++++++             | 72% ~18s          
   |+++++++++++++++++++++++++++++++++++++             | 73% ~17s          
   |++++++++++++++++++++++++++++++++++++++            | 74% ~16s          
   |++++++++++++++++++++++++++++++++++++++            | 75% ~16s          
   |+++++++++++++++++++++++++++++++++++++++           | 76% ~15s          
   |+++++++++++++++++++++++++++++++++++++++           | 77% ~14s          
   |++++++++++++++++++++++++++++++++++++++++          | 78% ~14s          
   |++++++++++++++++++++++++++++++++++++++++          | 79% ~13s          
   |+++++++++++++++++++++++++++++++++++++++++         | 80% ~12s          
   |+++++++++++++++++++++++++++++++++++++++++         | 81% ~12s          
   |++++++++++++++++++++++++++++++++++++++++++        | 82% ~11s          
   |++++++++++++++++++++++++++++++++++++++++++        | 84% ~10s          
   |+++++++++++++++++++++++++++++++++++++++++++       | 85% ~10s          
   |+++++++++++++++++++++++++++++++++++++++++++       | 86% ~09s          
   |++++++++++++++++++++++++++++++++++++++++++++      | 87% ~09s          
   |++++++++++++++++++++++++++++++++++++++++++++      | 88% ~08s          
   |+++++++++++++++++++++++++++++++++++++++++++++     | 89% ~07s          
   |+++++++++++++++++++++++++++++++++++++++++++++     | 90% ~07s          
   |++++++++++++++++++++++++++++++++++++++++++++++    | 91% ~06s          
   |++++++++++++++++++++++++++++++++++++++++++++++    | 92% ~05s          
   |+++++++++++++++++++++++++++++++++++++++++++++++   | 93% ~05s          
   |+++++++++++++++++++++++++++++++++++++++++++++++   | 94% ~04s          
   |++++++++++++++++++++++++++++++++++++++++++++++++  | 95% ~03s          
   |++++++++++++++++++++++++++++++++++++++++++++++++  | 96% ~03s          
   |+++++++++++++++++++++++++++++++++++++++++++++++++ | 97% ~02s          
   |+++++++++++++++++++++++++++++++++++++++++++++++++ | 98% ~01s          
   |++++++++++++++++++++++++++++++++++++++++++++++++++| 99% ~01s          
   |++++++++++++++++++++++++++++++++++++++++++++++++++| 100% elapsed = 01m 03s

   |                                                  | 0 % ~calculating  
   |+                                                 | 1 % ~01m 39s      
   |++                                                | 2 % ~01m 39s      
   |++                                                | 3 % ~01m 38s      
   |+++                                               | 4 % ~01m 38s      
   |+++                                               | 5 % ~01m 37s      
   |++++                                              | 6 % ~01m 36s      
   |++++                                              | 7 % ~01m 35s      
   |+++++                                             | 8 % ~01m 34s      
   |+++++                                             | 9 % ~01m 33s      
   |++++++                                            | 10% ~01m 32s      
   |++++++                                            | 11% ~01m 31s      
   |+++++++                                           | 12% ~01m 30s      
   |+++++++                                           | 13% ~01m 29s      
   |++++++++                                          | 14% ~01m 28s      
   |++++++++                                          | 15% ~01m 27s      
   |+++++++++                                         | 16% ~01m 26s      
   |+++++++++                                         | 17% ~01m 25s      
   |++++++++++                                        | 18% ~01m 24s      
   |++++++++++                                        | 19% ~01m 23s      
   |+++++++++++                                       | 20% ~01m 22s      
   |+++++++++++                                       | 21% ~01m 21s      
   |++++++++++++                                      | 22% ~01m 20s      
   |++++++++++++                                      | 23% ~01m 19s      
   |+++++++++++++                                     | 24% ~01m 18s      
   |+++++++++++++                                     | 25% ~01m 17s      
   |++++++++++++++                                    | 26% ~01m 16s      
   |++++++++++++++                                    | 27% ~01m 15s      
   |+++++++++++++++                                   | 28% ~01m 14s      
   |+++++++++++++++                                   | 29% ~01m 13s      
   |++++++++++++++++                                  | 30% ~01m 12s      
   |++++++++++++++++                                  | 31% ~01m 11s      
   |+++++++++++++++++                                 | 32% ~01m 10s      
   |+++++++++++++++++                                 | 33% ~01m 09s      
   |++++++++++++++++++                                | 34% ~01m 08s      
   |++++++++++++++++++                                | 35% ~01m 06s      
   |+++++++++++++++++++                               | 36% ~01m 05s      
   |+++++++++++++++++++                               | 37% ~01m 04s      
   |++++++++++++++++++++                              | 38% ~01m 03s      
   |++++++++++++++++++++                              | 39% ~01m 02s      
   |+++++++++++++++++++++                             | 40% ~01m 01s      
   |+++++++++++++++++++++                             | 41% ~01m 00s      
   |++++++++++++++++++++++                            | 42% ~59s          
   |++++++++++++++++++++++                            | 43% ~58s          
   |+++++++++++++++++++++++                           | 44% ~57s          
   |+++++++++++++++++++++++                           | 45% ~56s          
   |++++++++++++++++++++++++                          | 46% ~55s          
   |++++++++++++++++++++++++                          | 47% ~54s          
   |+++++++++++++++++++++++++                         | 48% ~53s          
   |+++++++++++++++++++++++++                         | 49% ~52s          
   |++++++++++++++++++++++++++                        | 51% ~51s          
   |++++++++++++++++++++++++++                        | 52% ~50s          
   |+++++++++++++++++++++++++++                       | 53% ~49s          
   |+++++++++++++++++++++++++++                       | 54% ~48s          
   |++++++++++++++++++++++++++++                      | 55% ~47s          
   |++++++++++++++++++++++++++++                      | 56% ~46s          
   |+++++++++++++++++++++++++++++                     | 57% ~44s          
   |+++++++++++++++++++++++++++++                     | 58% ~43s          
   |++++++++++++++++++++++++++++++                    | 59% ~42s          
   |++++++++++++++++++++++++++++++                    | 60% ~41s          
   |+++++++++++++++++++++++++++++++                   | 61% ~40s          
   |+++++++++++++++++++++++++++++++                   | 62% ~39s          
   |++++++++++++++++++++++++++++++++                  | 63% ~38s          
   |++++++++++++++++++++++++++++++++                  | 64% ~37s          
   |+++++++++++++++++++++++++++++++++                 | 65% ~36s          
   |+++++++++++++++++++++++++++++++++                 | 66% ~35s          
   |++++++++++++++++++++++++++++++++++                | 67% ~34s          
   |++++++++++++++++++++++++++++++++++                | 68% ~33s          
   |+++++++++++++++++++++++++++++++++++               | 69% ~32s          
   |+++++++++++++++++++++++++++++++++++               | 70% ~31s          
   |++++++++++++++++++++++++++++++++++++              | 71% ~30s          
   |++++++++++++++++++++++++++++++++++++              | 72% ~29s          
   |+++++++++++++++++++++++++++++++++++++             | 73% ~28s          
   |+++++++++++++++++++++++++++++++++++++             | 74% ~27s          
   |++++++++++++++++++++++++++++++++++++++            | 75% ~26s          
   |++++++++++++++++++++++++++++++++++++++            | 76% ~25s          
   |+++++++++++++++++++++++++++++++++++++++           | 77% ~24s          
   |+++++++++++++++++++++++++++++++++++++++           | 78% ~23s          
   |++++++++++++++++++++++++++++++++++++++++          | 79% ~22s          
   |++++++++++++++++++++++++++++++++++++++++          | 80% ~21s          
   |+++++++++++++++++++++++++++++++++++++++++         | 81% ~20s          
   |+++++++++++++++++++++++++++++++++++++++++         | 82% ~19s          
   |++++++++++++++++++++++++++++++++++++++++++        | 83% ~18s          
   |++++++++++++++++++++++++++++++++++++++++++        | 84% ~17s          
   |+++++++++++++++++++++++++++++++++++++++++++       | 85% ~16s          
   |+++++++++++++++++++++++++++++++++++++++++++       | 86% ~14s          
   |++++++++++++++++++++++++++++++++++++++++++++      | 87% ~13s          
   |++++++++++++++++++++++++++++++++++++++++++++      | 88% ~12s          
   |+++++++++++++++++++++++++++++++++++++++++++++     | 89% ~11s          
   |+++++++++++++++++++++++++++++++++++++++++++++     | 90% ~10s          
   |++++++++++++++++++++++++++++++++++++++++++++++    | 91% ~09s          
   |++++++++++++++++++++++++++++++++++++++++++++++    | 92% ~08s          
   |+++++++++++++++++++++++++++++++++++++++++++++++   | 93% ~07s          
   |+++++++++++++++++++++++++++++++++++++++++++++++   | 94% ~06s          
   |++++++++++++++++++++++++++++++++++++++++++++++++  | 95% ~05s          
   |++++++++++++++++++++++++++++++++++++++++++++++++  | 96% ~04s          
   |+++++++++++++++++++++++++++++++++++++++++++++++++ | 97% ~03s          
   |+++++++++++++++++++++++++++++++++++++++++++++++++ | 98% ~02s          
   |++++++++++++++++++++++++++++++++++++++++++++++++++| 99% ~01s          
   |++++++++++++++++++++++++++++++++++++++++++++++++++| 100% elapsed = 01m 42s

   |                                                  | 0 % ~calculating  
   |+                                                 | 1 % ~01m 33s      
   |++                                                | 2 % ~01m 32s      
   |++                                                | 3 % ~01m 31s      
   |+++                                               | 4 % ~01m 30s      
   |+++                                               | 5 % ~01m 29s      
   |++++                                              | 6 % ~01m 29s      
   |++++                                              | 7 % ~01m 28s      
   |+++++                                             | 8 % ~01m 27s      
   |+++++                                             | 9 % ~01m 27s      
   |++++++                                            | 10% ~01m 26s      
   |++++++                                            | 11% ~01m 25s      
   |+++++++                                           | 12% ~01m 24s      
   |+++++++                                           | 13% ~01m 23s      
   |++++++++                                          | 14% ~01m 22s      
   |++++++++                                          | 15% ~01m 21s      
   |+++++++++                                         | 16% ~01m 20s      
   |+++++++++                                         | 17% ~01m 19s      
   |++++++++++                                        | 18% ~01m 18s      
   |++++++++++                                        | 19% ~01m 17s      
   |+++++++++++                                       | 20% ~01m 16s      
   |+++++++++++                                       | 21% ~01m 15s      
   |++++++++++++                                      | 22% ~01m 14s      
   |++++++++++++                                      | 23% ~01m 14s      
   |+++++++++++++                                     | 24% ~01m 13s      
   |+++++++++++++                                     | 25% ~01m 12s      
   |++++++++++++++                                    | 26% ~01m 11s      
   |++++++++++++++                                    | 27% ~01m 10s      
   |+++++++++++++++                                   | 28% ~01m 09s      
   |+++++++++++++++                                   | 29% ~01m 08s      
   |++++++++++++++++                                  | 30% ~01m 07s      
   |++++++++++++++++                                  | 31% ~01m 06s      
   |+++++++++++++++++                                 | 32% ~01m 05s      
   |+++++++++++++++++                                 | 33% ~01m 04s      
   |++++++++++++++++++                                | 34% ~01m 03s      
   |++++++++++++++++++                                | 35% ~01m 02s      
   |+++++++++++++++++++                               | 36% ~01m 01s      
   |+++++++++++++++++++                               | 37% ~60s          
   |++++++++++++++++++++                              | 38% ~59s          
   |++++++++++++++++++++                              | 39% ~58s          
   |+++++++++++++++++++++                             | 40% ~57s          
   |+++++++++++++++++++++                             | 41% ~56s          
   |++++++++++++++++++++++                            | 42% ~55s          
   |++++++++++++++++++++++                            | 43% ~54s          
   |+++++++++++++++++++++++                           | 44% ~53s          
   |+++++++++++++++++++++++                           | 45% ~52s          
   |++++++++++++++++++++++++                          | 46% ~51s          
   |++++++++++++++++++++++++                          | 47% ~50s          
   |+++++++++++++++++++++++++                         | 48% ~49s          
   |+++++++++++++++++++++++++                         | 49% ~48s          
   |++++++++++++++++++++++++++                        | 51% ~47s          
   |++++++++++++++++++++++++++                        | 52% ~46s          
   |+++++++++++++++++++++++++++                       | 53% ~45s          
   |+++++++++++++++++++++++++++                       | 54% ~44s          
   |++++++++++++++++++++++++++++                      | 55% ~43s          
   |++++++++++++++++++++++++++++                      | 56% ~43s          
   |+++++++++++++++++++++++++++++                     | 57% ~42s          
   |+++++++++++++++++++++++++++++                     | 58% ~41s          
   |++++++++++++++++++++++++++++++                    | 59% ~40s          
   |++++++++++++++++++++++++++++++                    | 60% ~39s          
   |+++++++++++++++++++++++++++++++                   | 61% ~38s          
   |+++++++++++++++++++++++++++++++                   | 62% ~37s          
   |++++++++++++++++++++++++++++++++                  | 63% ~36s          
   |++++++++++++++++++++++++++++++++                  | 64% ~35s          
   |+++++++++++++++++++++++++++++++++                 | 65% ~34s          
   |+++++++++++++++++++++++++++++++++                 | 66% ~33s          
   |++++++++++++++++++++++++++++++++++                | 67% ~32s          
   |++++++++++++++++++++++++++++++++++                | 68% ~31s          
   |+++++++++++++++++++++++++++++++++++               | 69% ~30s          
   |+++++++++++++++++++++++++++++++++++               | 70% ~29s          
   |++++++++++++++++++++++++++++++++++++              | 71% ~28s          
   |++++++++++++++++++++++++++++++++++++              | 72% ~27s          
   |+++++++++++++++++++++++++++++++++++++             | 73% ~26s          
   |+++++++++++++++++++++++++++++++++++++             | 74% ~25s          
   |++++++++++++++++++++++++++++++++++++++            | 75% ~24s          
   |++++++++++++++++++++++++++++++++++++++            | 76% ~23s          
   |+++++++++++++++++++++++++++++++++++++++           | 77% ~22s          
   |+++++++++++++++++++++++++++++++++++++++           | 78% ~21s          
   |++++++++++++++++++++++++++++++++++++++++          | 79% ~20s          
   |++++++++++++++++++++++++++++++++++++++++          | 80% ~19s          
   |+++++++++++++++++++++++++++++++++++++++++         | 81% ~18s          
   |+++++++++++++++++++++++++++++++++++++++++         | 82% ~17s          
   |++++++++++++++++++++++++++++++++++++++++++        | 83% ~16s          
   |++++++++++++++++++++++++++++++++++++++++++        | 84% ~15s          
   |+++++++++++++++++++++++++++++++++++++++++++       | 85% ~14s          
   |+++++++++++++++++++++++++++++++++++++++++++       | 86% ~14s          
   |++++++++++++++++++++++++++++++++++++++++++++      | 87% ~13s          
   |++++++++++++++++++++++++++++++++++++++++++++      | 88% ~12s          
   |+++++++++++++++++++++++++++++++++++++++++++++     | 89% ~11s          
   |+++++++++++++++++++++++++++++++++++++++++++++     | 90% ~10s          
   |++++++++++++++++++++++++++++++++++++++++++++++    | 91% ~09s          
   |++++++++++++++++++++++++++++++++++++++++++++++    | 92% ~08s          
   |+++++++++++++++++++++++++++++++++++++++++++++++   | 93% ~07s          
   |+++++++++++++++++++++++++++++++++++++++++++++++   | 94% ~06s          
   |++++++++++++++++++++++++++++++++++++++++++++++++  | 95% ~05s          
   |++++++++++++++++++++++++++++++++++++++++++++++++  | 96% ~04s          
   |+++++++++++++++++++++++++++++++++++++++++++++++++ | 97% ~03s          
   |+++++++++++++++++++++++++++++++++++++++++++++++++ | 98% ~02s          
   |++++++++++++++++++++++++++++++++++++++++++++++++++| 99% ~01s          
   |++++++++++++++++++++++++++++++++++++++++++++++++++| 100% elapsed = 01m 36s

   |                                                  | 0 % ~calculating  
   |+                                                 | 1 % ~01m 30s      
   |++                                                | 2 % ~01m 29s      
   |++                                                | 3 % ~01m 29s      
   |+++                                               | 4 % ~01m 28s      
   |+++                                               | 5 % ~01m 27s      
   |++++                                              | 6 % ~01m 26s      
   |++++                                              | 7 % ~01m 28s      
   |+++++                                             | 8 % ~01m 27s      
   |+++++                                             | 9 % ~01m 26s      
   |++++++                                            | 10% ~01m 25s      
   |++++++                                            | 11% ~01m 24s      
   |+++++++                                           | 12% ~01m 23s      
   |+++++++                                           | 13% ~01m 22s      
   |++++++++                                          | 14% ~01m 21s      
   |++++++++                                          | 15% ~01m 20s      
   |+++++++++                                         | 16% ~01m 19s      
   |+++++++++                                         | 18% ~01m 18s      
   |++++++++++                                        | 19% ~01m 17s      
   |++++++++++                                        | 20% ~01m 16s      
   |+++++++++++                                       | 21% ~01m 15s      
   |+++++++++++                                       | 22% ~01m 14s      
   |++++++++++++                                      | 23% ~01m 13s      
   |++++++++++++                                      | 24% ~01m 12s      
   |+++++++++++++                                     | 25% ~01m 11s      
   |+++++++++++++                                     | 26% ~01m 10s      
   |++++++++++++++                                    | 27% ~01m 09s      
   |++++++++++++++                                    | 28% ~01m 08s      
   |+++++++++++++++                                   | 29% ~01m 07s      
   |+++++++++++++++                                   | 30% ~01m 06s      
   |++++++++++++++++                                  | 31% ~01m 05s      
   |++++++++++++++++                                  | 32% ~01m 04s      
   |+++++++++++++++++                                 | 33% ~01m 03s      
   |++++++++++++++++++                                | 34% ~01m 02s      
   |++++++++++++++++++                                | 35% ~01m 01s      
   |+++++++++++++++++++                               | 36% ~60s          
   |+++++++++++++++++++                               | 37% ~59s          
   |++++++++++++++++++++                              | 38% ~58s          
   |++++++++++++++++++++                              | 39% ~57s          
   |+++++++++++++++++++++                             | 40% ~56s          
   |+++++++++++++++++++++                             | 41% ~55s          
   |++++++++++++++++++++++                            | 42% ~54s          
   |++++++++++++++++++++++                            | 43% ~53s          
   |+++++++++++++++++++++++                           | 44% ~52s          
   |+++++++++++++++++++++++                           | 45% ~51s          
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head(tiss.markers)

Display the top markers you computed above.

tiss.markers %>% group_by(cluster) %>% top_n(5, avg_diff)

Assigning cell type identity to clusters

At a coarse level, we can use canonical markers to match the unbiased clustering to known cell types:

0: alpha 1: beta 2: beta 3: exocrine 4: duct 5: delta 6: gamma 7: endothelial 8: immune 9: stellate

Checking for batch effects

Color by metadata, like plate barcode, to check for batch effects.

Print a table showing the count of cells in each identity category from each plate.

Subset and iterate

We can repeat the above analysis on a subset of cells, defined using cluster IDs or some other metadata. This is a good way to drill down and find substructure.

Subset == T and NK cells (Cluster 7)

Run Principal Component Analysis.

Later on (in FindClusters and TSNE) you will pick a number of principal components to use. This has the effect of keeping the major directions of variation in the data and, ideally, supressing noise. There is no correct answer to the number to use, but a decent rule of thumb is to go until the plot plateaus.

Choose the number of principal components to use.

The clustering is performed based on a nearest neighbors graph. Cells that have similar expression will be joined together. The Louvain algorithm looks for groups of cells with high modularity–more connections within the group than between groups. The resolution parameter determines the scale…higher resolution will give more clusters, lower resolution will give fewer.

To visualize

Check expression of genes of interset.

Dotplots let you see the intensity of exppression and the fraction of cells expressing for each of your genes of interest.

How big are the clusters?

Checking for batch effects

Color by metadata, like plate barcode, to check for batch effects.

Print a table showing the count of cells in each identity category from each plate.

Assigning subannotations

For the subsets, we produce subannotations. These will be written back as metadata in the original object, so we can see all subannotations together.

If some of the clusters you find in the subset deserve additional annotation, you can add that right here. Use NA for clusters for which no subannotation is needed.

When you save the subtissue, please give it a name.

Export the final metadata

So that Biohub can easily combine all your annotations, please export them as a simple csv.

---
title: "Marrow FACS Notebook"
output:
  html_document: default
  html_notebook: default
---

Enter the directory of the maca folder on your drive and the name of the tissue you want to analyze.

```{r}
tissue_of_interest = "Marrow"
```

Load the requisite packages and some additional helper functions.

```{r}
library(here)
library(useful)
library(Seurat)
library(dplyr)
library(Matrix)

save_dir = here('00_data_ingest', 'tissue_robj')
```



```{r}
# read the metadata to get the plates we want
plate_metadata_filename = here('00_data_ingest', 'facs_raw_data', 'metadata_FACS.csv')

plate_metadata <- read.csv(plate_metadata_filename, sep=",", header = TRUE)
colnames(plate_metadata)[1] <- "plate.barcode"
plate_metadata
```

Subset the metadata on the tissue.

```{r}
tissue_plates = filter(plate_metadata, tissue == tissue_of_interest)[,c('plate.barcode','tissue','subtissue','mouse.sex')]
tissue_plates
```

Load the read count data.
```{r}
#Load the gene names and set the metadata columns by opening the first file
filename = here('00_data_ingest', 'facs_raw_data', 'FACS', paste0(tissue_of_interest, '-counts.csv'))

raw.data = read.csv(filename, sep=",", row.names=1)
# raw.data = data.frame(row.names = rownames(raw.data))
corner(raw.data)
```
Make a vector of plate barcodes for each cell

```{r}
plate.barcodes = lapply(colnames(raw.data), function(x) strsplit(strsplit(x, "_")[[1]][1], '.', fixed=TRUE)[[1]][2])
head(plate.barcodes)
```

Use only the metadata rows corresponding to Bladder plates. Make a plate barcode dataframe to "expand" the per-plate metadata to be per-cell.
```{r}
barcode.df = t.data.frame(as.data.frame(plate.barcodes))

rownames(barcode.df) = colnames(raw.data)
colnames(barcode.df) = c('plate.barcode')
head(barcode.df)

rnames = row.names(barcode.df)
meta.data <- merge(barcode.df, plate_metadata, by='plate.barcode', sort = F)
row.names(meta.data) <- rnames

# Sort cells by plate barcode because that's how the data was originally
meta.data = meta.data[order(meta.data$plate.barcode), ]
corner(meta.data)
raw.data = raw.data[, rownames(meta.data)]
corner(raw.data)
```
Process the raw data and load it into the Seurat object.

```{r}
# Find ERCC's, compute the percent ERCC, and drop them from the raw data.
erccs <- grep(pattern = "^ERCC-", x = rownames(x = raw.data), value = TRUE)
percent.ercc <- Matrix::colSums(raw.data[erccs, ])/Matrix::colSums(raw.data)
ercc.index <- grep(pattern = "^ERCC-", x = rownames(x = raw.data), value = FALSE)
raw.data <- raw.data[-ercc.index,]

# Create the Seurat object with all the data
tiss <- CreateSeuratObject(raw.data = raw.data, project = tissue_of_interest, 
                    min.cells = 5, min.genes = 5)

tiss <- AddMetaData(object = tiss, meta.data)
tiss <- AddMetaData(object = tiss, percent.ercc, col.name = "percent.ercc")
# Change default name for sums of counts from nUMI to nReads
colnames(tiss@meta.data)[colnames(tiss@meta.data) == 'nUMI'] <- 'nReads'

# Create metadata columns for annotations and subannotations
tiss@meta.data[,'annotation'] <- NA
tiss@meta.data[,'subannotation'] <- NA
```


Calculate percent ribosomal genes.

```{r}
ribo.genes <- grep(pattern = "^Rp[sl][[:digit:]]", x = rownames(x = tiss@data), value = TRUE)
percent.ribo <- Matrix::colSums(tiss@raw.data[ribo.genes, ])/Matrix::colSums(tiss@raw.data)
tiss <- AddMetaData(object = tiss, metadata = percent.ribo, col.name = "percent.ribo")
```

A sanity check: genes per cell vs reads per cell.

```{r}
GenePlot(object = tiss, gene1 = "nReads", gene2 = "nGene", use.raw=T)
```

Filter out cells with few reads and few genes.

```{r}
tiss <- FilterCells(object = tiss, subset.names = c("nGene", "nReads"), 
    low.thresholds = c(500, 50000), high.thresholds = c(25000, 2000000))
```


Normalize the data, then regress out correlation with total reads
```{r}
tiss <- NormalizeData(object = tiss)
tiss <- ScaleData(object = tiss, vars.to.regress = c("nReads", "percent.ribo","Rn45s"))
tiss <- FindVariableGenes(object = tiss, do.plot = TRUE, x.high.cutoff = Inf, y.cutoff = 0.5)
```


Run Principal Component Analysis.
```{r}
tiss <- RunPCA(object = tiss, do.print = FALSE)
tiss <- ProjectPCA(object = tiss, do.print = FALSE)
```

```{r, echo=FALSE, fig.height=4, fig.width=8}
PCHeatmap(object = tiss, pc.use = 1:3, cells.use = 500, do.balanced = TRUE, label.columns = FALSE, num.genes = 8)
```

Later on (in FindClusters and TSNE) you will pick a number of principal components to use. This has the effect of keeping the major directions of variation in the data and, ideally, supressing noise. There is no correct answer to the number to use, but a decent rule of thumb is to go until the plot plateaus.

```{r}
PCElbowPlot(object = tiss)
```

Choose the number of principal components to use.
```{r}
# Set number of principal components. 
n.pcs = 15
```


The clustering is performed based on a nearest neighbors graph. Cells that have similar expression will be joined together. The Louvain algorithm looks for groups of cells with high modularity--more connections within the group than between groups. The resolution parameter determines the scale...higher resolution will give more clusters, lower resolution will give fewer.

For the top-level clustering, aim to under-cluster instead of over-cluster. It will be easy to subset groups and further analyze them below.

```{r}
# Set resolution 
res.used <- 0.5

tiss <- FindClusters(object = tiss, reduction.type = "pca", dims.use = 1:n.pcs, 
    resolution = res.used, print.output = 0, save.SNN = TRUE)
```

To visualize 
```{r}
# If cells are too spread out, you can raise the perplexity. If you have few cells, try a lower perplexity (but never less than 10).
tiss <- RunTSNE(object = tiss, dims.use = 1:n.pcs, seed.use = 10, perplexity=84)
```

```{r}
# note that you can set do.label=T to help label individual clusters
TSNEPlot(object = tiss, do.label = T)
```

Check expression of genes of interset.

```{r, echo=FALSE, fig.height=84, fig.width=15}
genes_to_check = c('Itgam', 'Il7r', 'Kit', 'Atxn1', 'Fcgr3', 'Flt3', 'Cd34', 'Slamf1', 'Gpr56', 'Stmn1', 'Tmem176b',  'Itgal', 'Itgax', 'Emr1', 'Cd68', 'Fcgr4', 'Mpeg1', 'Itgb2', 'Ahnak', 'Pld4', 'Cd3e', 'Cd4', 'Cd8a', 'Ly6d', 'Cd27', 'Cr2', 'Fcer2a', 'Cd2', 'Cd7', 'Mme', 'Thy1', 'Cd19', 'Ms4a1', 'Cd74', 'Chchd10', 'Cnp', 'Cd79a', 'Cd79b', 'Vpreb3', 'Klrb1a', 'S100a11', 'Ltf', 'Ngp', 'Fcer1g', 'Pglyrp1', 'Lcn2', 'Camp', 'Hp', 'Ly6g6c', 'Ly6g6e', 'Ptprc')
#genes_to_check = c('Alb', 'Cyp2f2', 'Cyp2e1', 'Hamp')

FeaturePlot(tiss, genes_to_check, pt.size = 1, nCol = 3)
```

Dotplots let you see the intensity of exppression and the fraction of cells expressing for each of your genes of interest.

```{r, echo=FALSE, fig.height=4, fig.width=39}
# To change the y-axis to show raw counts, add use.raw = T.
DotPlot(tiss, genes_to_check, plot.legend = T)
```

How big are the clusters?
```{r}
table(tiss@ident)
```

Which markers identify a specific cluster?

```{r}
clust.markers <- FindMarkers(object = tiss, ident.1 = 0, only.pos = TRUE, min.pct = 0.25, thresh.use = 0.25)
```

```{r}
print(x = head(x= clust.markers, n = 10))
```

You can also compute all markers for all clusters at once. This may take some time.
```{r}
tiss.markers <- FindAllMarkers(object = tiss, only.pos = TRUE, min.pct = 0.25, thresh.use = 0.25)
```
```{r}
head(tiss.markers)
```

Display the top markers you computed above.
```{r}
tiss.markers %>% group_by(cluster) %>% top_n(5, avg_diff)
```

## Assigning cell type identity to clusters

At a coarse level, we can use canonical markers to match the unbiased clustering to known cell types:

0: alpha
1: beta
2: beta
3: exocrine
4: duct
5: delta
6: gamma
7: endothelial
8: immune
9: stellate

```{r}
# stash current cluster IDs
tiss <- StashIdent(object = tiss, save.name = "cluster.ids")

# enumerate current cluster IDs and the labels for them
cluster.ids <- c(0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10)
annotation <- c("hematopoietic stem cell", "B cell", "B cell", "neutrophil", "granulocyte", "monocyte", "B cell", "T_NK", "neutrophil", "Fraction A pre-pro B cell", "hematopoietic stem cell")
cell_ontology_id <- c("CL:0000037", "CL:0000236", "CL:0000236", "CL:0000775", "CL:0000094", "CL:0000576", "CL:0000236", NA, "CL:0000775", "CL:0002045", "CL:0000037")
  

tiss@meta.data[,'annotation'] <- plyr::mapvalues(x = tiss@ident, from = cluster.ids, to = annotation)
tiss@meta.data[,'cell_ontology_id'] <- plyr::mapvalues(x = tiss@ident, from = cluster.ids, to = cell_ontology_id)

tiss@meta.data[tiss@cell.names,'annotation'] <- as.character(tiss@meta.data$annotation)
tiss@meta.data[tiss@cell.names,'cell_ontology_id'] <- as.character(tiss@meta.data$cell_ontology_id)


TSNEPlot(object = tiss, do.label = TRUE, pt.size = 0.5, group.by='annotation')
```


## Checking for batch effects


Color by metadata, like plate barcode, to check for batch effects.
```{r}
TSNEPlot(object = tiss, do.return = TRUE, group.by = "plate.barcode")
```

Print a table showing the count of cells in each identity category from each plate.

```{r}
table(as.character(tiss@ident), as.character(tiss@meta.data$plate.barcode))
```


# Subset and iterate

We can repeat the above analysis on a subset of cells, defined using cluster IDs or some other metadata. This is a good way to drill down and find substructure.


## Subset == T and NK cells (Cluster 7)

```{r}
# Subset data based on cluster id
subtiss7 <- SubsetData(object = tiss, ident.use = c(7), do.center = F, do.scale = F, cells.use = )

# To subset data based on annotation or other metadata, you can explicitly pass cell names

# anno = 'exocrine cells'
# cells.to.use = tiss@cell.names[which(tiss@meta.data$annotation == anno)]
# subtiss7 <- SubsetData(object = tiss, cells.use = cells.to.use, do.center = F, do.scale = F)

```

```{r}
subtiss7 <- NormalizeData(object = subtiss7)
subtiss7 <- ScaleData(object = subtiss7, vars.to.regress = c("nReads", "percent.ribo","Rn45s"))
```

```{r}
subtiss7 <- FindVariableGenes(object = subtiss7, do.plot = TRUE, x.high.cutoff = Inf, y.cutoff = 0.8)
subtiss7 <- RunPCA(object = subtiss7, pcs.compute = 20)
subtiss7 <- ProjectPCA(object = subtiss7, do.print = FALSE)
```


Run Principal Component Analysis.
```{r}
subtiss7 <- RunPCA(object = subtiss7, do.print = FALSE)
subtiss7 <- ProjectPCA(object = subtiss7, do.print = FALSE)
```

```{r}
# If this fails for your subset, it may be that cells.use is more cells than you have left! Try reducing it.
PCHeatmap(object = subtiss7, pc.use = 1:3, cells.use = 250, do.balanced = TRUE, label.columns = FALSE, num.genes = 12)
```

Later on (in FindClusters and TSNE) you will pick a number of principal components to use. This has the effect of keeping the major directions of variation in the data and, ideally, supressing noise. There is no correct answer to the number to use, but a decent rule of thumb is to go until the plot plateaus.

```{r}
PCElbowPlot(object = subtiss7)
```

Choose the number of principal components to use.
```{r}
# Set number of principal components. 
sub7.n.pcs = 5
```


The clustering is performed based on a nearest neighbors graph. Cells that have similar expression will be joined together. The Louvain algorithm looks for groups of cells with high modularity--more connections within the group than between groups. The resolution parameter determines the scale...higher resolution will give more clusters, lower resolution will give fewer.

```{r}
# Set resolution 
sub.res.used <- 1

subtiss7 <- FindClusters(object = subtiss7, reduction.type = "pca", dims.use = 1:sub7.n.pcs, 
    resolution = sub.res.used, print.output = 0, save.SNN = TRUE)
```

To visualize 
```{r}
# If cells are too spread out, you can raise the perplexity. If you have few cells, try a lower perplexity (but never less than 10).
subtiss7 <- RunTSNE(object = subtiss7, dims.use = 1:sub7.n.pcs, seed.use = 10, perplexity=30)
```

```{r}
# note that you can set do.label=T to help label individual clusters
TSNEPlot(object = subtiss7, do.label = T)
```

```{r}
subtiss7.markers <- FindAllMarkers(object = subtiss7, only.pos = TRUE, min.pct = 0.25, thresh.use = 0.25)
```

```{r}
subtiss7.markers %>% group_by(cluster) %>% top_n(6, avg_diff)
```

Check expression of genes of interset.
```{r, echo=FALSE, fig.height=48, fig.width=15}
genes_to_check = c('Cd6','Il7r','Ctla4','Cd8b1', 'Cxcr6', 'Cd8a', 'Tyrobp', 'Ncr1', 'Cd3e', 'Klrb1a', 'Klrb1c', 'Gzma', 'Prf1', 'Serpinb9', 'Lyz2', 'Ngp', 'Hp', 'Ly6c2', 'Cd79a', 'Cd74', 'H2-Aa', 'H2-Ab1', 'Cd79b', 'H2-Eb1', 'Ccna2', 'Stmn1', 'Top2a', 'Mki67', 'Rrm2', 'Nkg7')

FeaturePlot(subtiss7, genes_to_check, pt.size = 1, nCol = 3)
```

Dotplots let you see the intensity of exppression and the fraction of cells expressing for each of your genes of interest.

```{r, echo=FALSE, fig.height=4, fig.width=30}
# To change the y-axis to show raw counts, add use.raw = T.
DotPlot(subtiss7, genes_to_check, plot.legend = T)
```

How big are the clusters?
```{r}
table(subtiss7@ident)
```

## Checking for batch effects

Color by metadata, like plate barcode, to check for batch effects.
```{r}
TSNEPlot(object = subtiss7, do.return = TRUE, group.by = "plate.barcode")
```

Print a table showing the count of cells in each identity category from each plate.

```{r}
table(as.character(subtiss7@ident), as.character(subtiss7@meta.data$plate.barcode))
```



### Assigning subannotations

For the subsets, we produce subannotations. These will be written back as metadata in the original object, so we can see all subannotations together.

If some of the clusters you find in the subset deserve additional annotation, you can add that right here. Use NA for clusters for which no subannotation is needed.

```{r}
subcluster.ids <- c(0, 1, 2, 3, 4, 5)
subannotation <- c("T cell","natural killer cell", "natural killer cell", "T cell", "T cell", "T cell")
cell_ontology_id = c("CL:0000084", "CL:0000623", "CL:0000623", "CL:0000084", "CL:0000084", "CL:0000084")

subtiss7@meta.data[,'annotation'] <- plyr::mapvalues(x = subtiss7@ident, from = subcluster.ids, to = annotation)
subtiss7@meta.data[,'cell_ontology_id'] <- plyr::mapvalues(x = subtiss7@ident, from = subcluster.ids, to = cell_ontology_id)

tiss@meta.data[subtiss7@cell.names,'annotation'] <- as.character(subtiss7@meta.data$annotation)
tiss@meta.data[subtiss7@cell.names,'cell_ontology_id'] <- as.character(subtiss7@meta.data$cell_ontology_id)

TSNEPlot(object = subtiss7, do.label = TRUE, pt.size = 0.5, group.by='annotation')
```

When you save the subtissue, please give it a name.

```{r}
subtiss7.name = 'Nk_T_cells'
save(subtiss7, file=paste0(save_dir,"/",subtiss7.name, "_seurat_subtiss7.Robj"))
```


# Export the final metadata

So that Biohub can easily combine all your annotations, please export them as a simple csv.

```{r}
write.csv(tiss@meta.data[,c('plate.barcode','annotation','subannotation')],file =paste0(save_dir,"/", tissue_of_interest,"_annotation.csv"))
```



